Now compatible with innovation
why is the Earth abundant in diversity? The secret lies in the functioning of organisms' genetic makeup. Unfortunately, scholars understand little of it, and this slows down the process of conserving ever-dwindling biodiversity.
Recently, a breakthrough has been made. A research team from the us-based Pennsylvania State University (psu) has discovered a gene that controls pollen functioning during the self-incompatibility process. Flowering plants prevent inbreeding and thereby increase their diversity via the self-incompatibility process. During the process, pollination fails to yield seeds if the pistil (female plant organ) identifies the pollen (dustlike grains containing the male fertilising element) as its own.
The pollen gene was the critical missing link in the understanding of how self-incompatibility works. Ten years ago, researchers from psu had identified the s-rnase gene, which controls pistil functioning. The recently identified gene has been named pislf. Apart from helping conservationists, its discovery could lead to the development of hybrids of crops such as corn and soybean, which have lost their self-incompatibility characteristics (Nature, Vol 429, No 6989, May 20, 2004).
How the gene functions Pollen grains of all plants are haploid. This implies they contain only one set of chromosomes, and each pollen grain comprises only one of the two self-incompatibility alleles (s-alleles) of the parent plant. An allele is one of the number of possible variants of a particular gene. For example, two alleles exist for each of the three genes that determine the eye colour in human beings.
The pistil is diploid, meaning it has two sets of chromosomes, and hence both s-alleles of the parent plant. During pollination, if the s-allele of the pollen does not match with either of the two s-alleles in the pistil, the pollen germinates on the surface of the pistil to produce pollen tubes, which then grow and lead to fertilisation. However, if the s-allele of the pollen matches with the two s-alleles in the pistil, growth of the pollen tube is hampered, preventing fertilisation from taking place.
To identify the pollen gene, the team examined the dna sequence of the chromosomal region containing the alleles of the pistil gene. "We did so, as the gene controlling the pollen function was likely to be closely located to the s-rnase gene to prevent recombination. A recombination between these two genes would cause the breakdown of the self-incompatibility process,' explains Teh-hui Kao, the lead researcher.
After years of scrutiny the team was able to locate the pislf gene. But the researchers had to demonstrate that pislf was indeed the pollen component of self-incompatibility. "Other labs have found similar genes in the vicinity of the s-rnase, but proximity alone is insufficient to establish the relationship.'
Undeniable proof To achieve their aim, the researchers relied on the competitive interaction phenomenon: if the pollen has two different alleles of the s-gene (which results
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